96 determinations of IgG antibodies using standard methodologies.
Wells sensitized with recombinant antigen (Em18) from Echinococcus multilocularis.
8-well polystyrene breakable strips : useful and economic format for
laboratories with a small workload.
CE registration: CH-201708-0010
Sensitivity and specificity
pre- and post-surgical serum samples of 12 patients with confirmed
alveolar echinococcosis and having had a radical or non-radical surgery
were studied. Pre-surgically, 9 patients (75%) had an index >1. Among
these patients, 5 had negative post-surgical results. But in all 12
patients, post-surgical Em18 antibody levels dropped and were
significantly lower than in pre-surgical samples.
samples of 25 patients with confirmed alveolar echinococcosis without
surgery but with stable disease under antiparasitic chemotherapy were
studied. 18 (72%) of them had an index >1 (median index 6.3).
samples of 7 patients with confirmed alveolar echinococcosis without
surgery but with progressive disease under antiparasitic chemotherapy were
studied. 6 (86%) of them had an index >1 (median index 13.8).
96 wells coated with Echinococcus multilocularis Em18
buffer (TBS-Tween), 50 ml
Washing solution, 50 ml
Enzyme buffer, 50 ml
Stop solution, 25 ml
Negative control serum (rabbit), 200 µl
Cut-off serum (rabbit), 200 µl
Positive control serum (rabbit), 200 µl
Protein A - AP conjugate, 300 µl
Alk. phosphatase substrate, 20 tab.
Multipipette resevoir 25ml, 1x
Frame for ELISA 8-wells holder, 1x
Instructions for use
#9310 Echinococcus multilocularis (Em18)
IgG ELISA (96 assays)
Ammann, R.W., Rebber, E.C.,
Gottstein, B., Grimm, F. Eckert, J., Renner, E.L. (2004)
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Tappe, D., Sako, Y., Itoh, S.
Frosch, M., Gruner, B., Kern, P., Ito, A. (2010)
Immunoglobulin G subclass responses to recombinant Em18 in the follow-up of
patients with alveolar echinococcosis in different clinical stage. Clin
Vaccine Immunol. 17 : 944-8.
Ammann, R.W., Stumpe, K.D.M.,
Grimm, F. Deplazes, P., Huber, S., Bertogg, K., et al. (2015)
Outcome after discontinuing long-term benzimidazole treatment in 11 patients
with non-resectable alveolar echinococcosis with negative FDG-PET/CT and
anti-EmII/3-10 serology. PloS
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