96 determinations of IgG antibodies using standard methodologies.
Wells sensitized with recombinant antigen (Em18) from Echinococcus multilocularis.
8-well polystyrene breakable strips : useful and economic format for
laboratories with a small workload.
CE registration: CH-201708-0010
Sensitivity and specificity
pre- and post-surgical serum samples of 12 patients with confirmed
alveolar echinococcosis and having had a radical or non-radical surgery
were studied. Pre-surgically, 9 patients (75%) had an index >1. Among
these patients, 5 had negative post-surgical results. But in all 12
patients, post-surgical Em18 antibody levels dropped and were
significantly lower than in pre-surgical samples.
samples of 25 patients with confirmed alveolar echinococcosis without
surgery but with stable disease under antiparasitic chemotherapy were
studied. 18 (72%) of them had an index >1 (median index 6.3).
samples of 7 patients with confirmed alveolar echinococcosis without
surgery but with progressive disease under antiparasitic chemotherapy were
studied. 6 (86%) of them had an index >1 (median index 13.8).
96 wells coated with Echinococcus multilocularis Em18
buffer (TBS-Tween), 50 ml
Washing solution, 50 ml
Enzyme buffer, 50 ml
Stop solution, 25 ml
Negative control serum (rabbit), 200 µl
Cut-off serum (rabbit), 200 µl
Positive control serum (rabbit), 200 µl
Protein A - AP conjugate, 300 µl
Alk. phosphatase substrate, 20 tab.
Multipipette resevoir 25ml, 1x
Frame for ELISA 8-wells holder, 1x
Instructions for use
Echinococcus multilocularis (Em18)
complete kit (96 assays) No. 9310
Ammann, R.W., Rebber, E.C.,
Gottstein, B., Grimm, F. Eckert, J., Renner, E.L. (2004)
Immunosurveillance of alveolar echinococcosis by specific humoral and
cellular immune tests: long-term analysis of the Swiss chemotherapy trial
(1976-2001). (2004) J. Hepatol. 41
D., Frosch, M., Sako, Y. Itoh, S., Gruner, B., Reuter, S., Nakao, M., Ito,
A., Kern, P. (2009) Close relationship between
clinical regression and specific serology in the follow-up of patients with
alveolar echinococcosis in different clinical stages. (2009)
Am. J. Trop. Med. Hyg. 80 : 792-7.
Y., Sako, Y. Itoh, S., Ohtake, T., Kohgo, Y., Matsuno, T., Ohsaki, Y.,
Miyokawa, N., Nakao, M., Nakaya, K., Ito, A.
(2009) Serological monitoring of progression of alveolar echinococcosis with
multiorgan involvement by use of recombinant Em18. J. Clin. Microbiol. 47
Tappe, D., Sako, Y., Itoh, S.
Frosch, M., Gruner, B., Kern, P., Ito, A. (2010)
Immunoglobulin G subclass responses to recombinant Em18 in the follow-up of
patients with alveolar echinococcosis in different clinical stage. Clin
Vaccine Immunol. 17 : 944-8.
Ammann, R.W., Stumpe, K.D.M.,
Grimm, F. Deplazes, P., Huber, S., Bertogg, K., et al. (2015)
Outcome after discontinuing long-term benzimidazole treatment in 11 patients
with non-resectable alveolar echinococcosis with negative FDG-PET/CT and
anti-EmII/3-10 serology. PloS
Negl Trop Dis. 9.