Toxocara canis
ELISA kit for the diagnosis of human toxocarosis

Parasitology and clinical relevance

Human toxocarosis (visceral larva migrans) is caused by an infection with the round worm Toxocara ssp. larvae. Dogs infected with adult worms release in their feces a large number of infectious eggs into the environment. After ingestion of eggs by the patient, the infecting larvae begin a somatic migration through the inner organs, releasing immunogenic compounds globally known as excretory/secretory (E/S) antigens. Clinical manifestations of toxocarosis include frequently a marked hypereosinophilia and also asthenia, abdominal pain or asthma. Larvae remain most frequently located in the liver and the lungs but can also cause serious ocular damage by migrating into the retina (ocular larva migrans). As the direct detection of larvae is not feasible in humans, a serological assay, on E/S antigens, is presently the most sensitive method available for the diagnosis of toxocarosis.

Test-Kit

96 determinations of IgG antibodies using standard methodologies

Wells sensitized with E/S antigens of cultured T. canis larvae. These antigens give to the test its high sensitivity and specificity.

8-wells polystyrene breakable strips : useful and economic format for laboratories with a small workload.
CE registration:  H-CH/CA01/IVD/01755

Sensitivity and specificity

Sensitivity > 90 %

Seroprevalence : 4 % on blood donors (Switzerland). Contacts without any clinical symptoms may have titers between the negative and the cut-off serums

Cross-reactivity with other helminthiases at titers lower than the positive control serum might occur

Contents

96 wells coated with Toxocara canis E/S antigens

TBS-Tween,  50 ml
Washing solution,  50 ml
Enzyme buffer,  50 ml
Stop solution,  25 ml
Negative control serum (human),  200 ml
Cut-off serum (human),  200 ml
Positive control serum (human),  200 ml
Anti-human IgG AP conjugate,  300 ml
Alk. Phosphatase substrate,  20 tablets
Multipipette reservoir
Frame for ELISA 8-wells holder
Detailed protocol

Ordering information

Toxocara canis
complete kit (96 assays) No. 9200

Toxocara canis
E/S antigens (96 assays) No. 9220

References

Jacquier, P. & al. (1991) J. Clin. Microbiol. 29, 1831-1835

Gueglio, B. & al. (1994) Parasitol. Res. 80, 531-536

Gems, D. & Maizel, R.M. (1996) Proc. Natl. Acad. Sci.  USA 93, 1665-1670

European directive 98/79/EC registration: Click here.     Material Safety Data Sheet: Click here.
Technical sheet and instructions for article N° 9200, PDF format, 23 Ko: Click here.

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