Echinococcus multilocularis
ELISA kit for the diagnosis of alveolar echinococcosis in humans

Parasitology and clinical relevance

Alveolar echinococcosis is caused by an infection with the cestode Echinococcus multilocularis and is a very dangerous disease for humans. Adult worms mature in the intestine of the definitive host (usually fox, occasionally dog or cat) and the eggs are released in the feces. Accidental ingestion of soil, berries or vegetables contaminated with eggs iniate the infection in humans. Oncospheres hatch in the duodenum, penetrate the intestine and are carried via the bloodstream to organs. Although alveolar hydatid cysts have been found in other tissues, the most common site of development is the liver. The cysts are not limited by a membrane but penetrate the tissues and may resemble a slow growing carcinoma. Surgical removing is difficult but drugs have been found that stop the development of the metacestode. Medical imaging and serology give both useful and complementary information on the nature of the cyst and on the outcome of treatment.

Test-Kit

96 determinations of IgG antibodies using standard methodologies

Wells sensitized with recombinant and affinity purified antigens (Em2-Em18) from Echinococcus multilocularis

8-well polystyrene breakable strips : useful and economic format for laboratories with a small workload

CE registration:  H-CH/CA01/IVD/01757

Sensitivity and specificity

Sensitivity

93% on alveolar echinococcosis
(E. multilocularis)

Specificity : 98 % negativity on blood donors (Switzerland)

Cross-reactivities :

10 % with helminthiases
26 % with protozooses 
~15 % with hydatidosis (E. granulosus)

Contents

96 wells coated with Echinococcus multilocularis Em2-Em18 antigens

TBS-Tween,  50 ml
Washing solution,  50 ml
Enzyme buffer,  50 ml
Stop solution,  25 ml
Negative control serum (rabbit),  200
ml
Cut-off serum (rabbit),  200
ml
Positive control serum (rabbit),  200
ml
Protein A - AP conjugate,  300
ml
Alk. Phosphatase substrate,  20 tablets
Multipipette reservoir
Frame for ELISA 8-wells holder
Detailed protocol

Ordering information

Echinococcus multilocularis,
complete kit (96 assays) No. 9300

References

Müller N. & al. (1989) Mol. Biochem. Parasitol. 36, 151-160

Gottstein B. & Jacquier P. (1991) Archivos de la Hidatidosis 30, 831-834

Gottstein B. & al. (1993) J. Clin. Microbiol. 31, 373-376

Müller N. & al. (2006) Parasitology, 134, 1-10

European directive 98/79/EC registration: Click here.   Material Safety Data Sheet: Click here.
Technical sheet and instructions for article N° 9300, PDF format, 24 Ko: Click here.

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