Echinococcus granulosus
ELISA kit for the diagnosis of echinococcoses in humans

Parasitology and clinical relevance

Cystic hydatid disease is caused by an infection with the cestode Echinococcus granulosus and is potentially dangerous for humans. New cases are mostly found in areas where dogs and livestock coexist. Adult worms mature in the intestine of the dog (definitive host) and the eggs are released in the feces. By an accidental ingestion in humans, oncospheres hatch in the duodenum, penetrate the intestine and are carrieds via the bloodstream to various organs. Hydatid cysts form in organs like liver (60-70 %), lungs and brain. The metacestode cyst develops over years in the patient and can, in most cases, be surgically removed after diagnosis. Medical imaging and serology give both usefull and complementary information on the nature of the cyst and concerning the issue of surgical intervention. The test uses an antigen preparation (hydatid fluid) which detects indiscriminately antibodies from patients with cystic hydatid disease (E. granulosus) or with alveolar echinococcosis (E. multilocularis).

Test-Kit

96 determinations of IgG antibodies using standard methodologies

Wells sensitized with antigens from Echinococcus granulosus hydatid fluid. The wide spectrum of antigens gives to the test a high sensitivity

8-wells polystyrene breakable strips : useful and economic format for laboratories with a small workload

CE registration:  H-CH/CA01/IVD/01757

Sensitivity and specificity

Sensitivities

96 % on cystic hydatid diseases (E. granulosus)
90 % on alveolar echinococcoses (E. multilocularis)

Specificity : 97 % negativity on blood donors (Switzerland)

Cross-reactivities :

18 % with helminthiases
17 % with protozooses 

In case of positive results, the use of the Em2-plus ELISA (No 9300) and a search for anti-8 kD band antibodies by western blot are recommended for a species-specific diagnosis

Contents

96 wells coated with Echinococcus granulosus antigens (hydatid fluid)

TBS-Tween, 50 ml
Washing solution, 50 ml
Enzyme buffer, 50 ml
Stop solution, 25 ml
Negative control serum (rabbit), 200 ml
Cut-off serum (rabbit), 200 ml
Positive control serum (rabbit), 200 ml
Protein A - Alk. phosphatase conjugate, 300 ml
Alk. phosphatase substrate, 20 tablets
Multipipette reservoir
Frame for ELISA 8-wells holder
Detailed protocol

Ordering information

Echinococcus granulosus
complete kit (96 assays) No. 9350

References

Gottstein, B. & al. (1993) J. Clin. Microbiol. 31, 373-376

Gottstein, B. (1992) Clin. Microbiol. Rev. 5, 248-261

European directive 98/79/EC registration: Click here.   Material Safety Data Sheet: Click here.
Technical sheet and instructions for article N° 9350, PDF format, 23 Ko: Click here.

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