Strongyloides ratti
ELISA kit for the diagnosis of strongyloidosis in humans

Parasitology and clinical relevance

Strongyloides stercoralis is a potentially dangerous parasite, particularly if unsuspected and present in immunocompromised patients or in patients receiving immunosuppressive drugs. An hyperinfection syndrome can occur with widespread nematode dissemination and a high mortality. Parasitological diagnosis is often time- consuming as Strongyloides larvae in the stool are frequently absent or present in very small numbers. Therefore, a serological assay to detect infection with Strongyloides is useful. Our ELISA uses the structural similarities existing between S. stercoralis and S. ratti antigens to detect antibodies in human sera.

Test-Kit

96 determinations of IgG antibodies using standard methodologies
Wells sensitized with somatic antigens from larvae of Strongyloides ratti.
8-wells polystyrene breakable strips: useful and economic format for laboratories with a small workload

CE registration: H-CH/CA01/IVD/10285

Sensitivity and specificity

Sensitivity 88 % with sera from patients with larvae of Strongyloides stercoralis.

Specificity: 94 % with sera of blood donors.

A specificity of 77 % was found with sera of patients with other parasitoses (mostly helminthiases).

Contents

96 wells coated with somatic antigens from larvae of Strongyloides ratti.
TBS-Tween 50 ml
Washing solution 50 ml
Enzyme buffer 50 ml
Stop solution 25 ml
Negative control serum (rabbit) 200 µl
Cut-off serum (rabbit) 200 µl
Positive control serum (rabbit) 200 µl
Protein A - AP conjugate 300 µl
Alk. phosphatase substrate 20 tab.
Multipipette resevoir 25ml 1x
Frame for ELISA 8-wells holder 1x
Detailed protocol 

Ordering information

Strongyloides ratti.
complete kit (96 assays) No. 9450

References

Magnaval, J.-F. et al. (2000) Eur. J. Epidemiol., 16, 179-182.

Schaffel, R. et al. (2001) Am. J. Trop. Med. Hyg., 65, 346-350.

Loutfy, M.R. et al. (2002) Am. J. Trop. Med. Hyg., 66, 749-752.

European directive 98/79/EC registration: Click here.   Material Safety Data Sheet: Click here.
Technical sheet and instructions for article N° 9450, PDF format, 24 Ko: Click here.
Assessment of a new ELISA test for the serodiagnosis of Strongyloidiasis (poster presentation): Click here

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