Schistosoma mansoni
ELISA kit for the diagnosis of schistosomiasis in humans

Parasitology and clinical relevance

Two hundred million people are suffering from schistosomiasis in sub-topical areas. Patients become infected when bathing in contaminated fresh water.
The disease is confirmed by microscopy when eggs are detected in stool or urine samples. However when the worm burden is light or during the invasive phase, few or no eggs are excreted. In such situations the serodiagnosis is a useful complement to parasitolgical tests.
The diagnosis of human schistosomiasis using the ELISA kit is intended for travelers with frequent contacts with fresh water returning from endemic areas, for travel companions of confirmed cases, to post therapeutic controls and to control programs in endemic areas.

Test-Kit

96 determinations of IgG antibodies using standard methodologies.

Wells sensitized with soluble antigens from Schistosoma mansoni.

8-wells polystyrene breakable strips: useful and economic format for laboratories with a small workload

CE registration: H-CH/CA01/IVD/17983

Sensitivity and specificity

The test detects both Schistosoma mansoni and S. haematobium infections.

A sensitivity of 94% was found with sera from patients with parasitologically proven Schistosoma mansoni and or Schistosoma haematobium infections

A specificity of 99 % was found with 122 sera of blood donors.

A specificity of 94% was found with patients with other parasitc infections.

Contents

96 wells coated with soluble antigens from Schistosoma mansoni.
TBS-Tween 50 ml
Washing solution 50 ml
Enzyme buffer 50 ml
Stop solution 25 ml
Negative control serum (rabbit) 200 µl
Cut-off serum (rabbit) 200 µl
Positive control serum (rabbit) 200 µl
Protein A - AP conjugate 300 µl
Alk. phosphatase substrate 20 tab.
Multipipette resevoir 25ml 1x
Frame for ELISA 8-wells holder 1x
Detailed protocol 

Ordering information

Schistosoma mansoni
complete kit (96 assays) No. 9600

References

Doenhoff, M.J. et al.(2003) Ann Trop Med Parasitol. 97:697-709.

Turner, P. et al. (2004) J Clin Pathol . 57 : 1193-1196.

Sorgho, H. et al. (2005) Acta Tropica. 93 : 169-180.

 

European directive 98/79/EC registration: Click here.   Material Safety Data Sheet: Click here.
Technical sheet and instructions for article N°9600, PDF format, 24 Ko: Click here.

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Click here to read long-term quality control reports

 

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