Acanthocheilonema viteae
ELISA kit for the diagnosis of filarioses in humans

Parasitology and clinical relevance

Human filarioses are caused by a variety of nematodes (Loa Loa, Onchocerca volvulus, Dracunculus medinensis, Wuchereria bancrofti, Brugia malayi). The diagnosis is based on clinical evidences, an history of living in an endemic area, hypereosinophilia and the detection of microfilariae. The finding of an adult filaria is a proof of infection but is frequently unsuccessful. Serological methods using indirect immunofluorescence assays on frozen sections of adult worms of animal origin (Acanthocheilonema viteae, Dirofilaria immitis, Setaria sp.) and immuno-electrophoresis can both help for diagnosis. These methods are often positive in cases of occult filarioses or when microfilaremia is negative, they are less sensitive in patients with detectable microfilariae. Our ELISA assay uses also the antigenic homologies observed between human and animal filariae. The ELISA procedure is more reproducible and less tedious than IFA on worm sections.

Test-Kit

96 determinations of IgG antibodies using standard methodologies
Wells sensitized with somatic antigens from adult females of Acanthocheilonema viteae.
8-wells polystyrene breakable strips: useful and economic format for laboratories with a small workload

CE registration: H-CH/CA01/IVD/01755

Sensitivity and specificity

Sensitivity 95 % with sera of suspected and confirmed cases of filarioses

Specificity: 98 %

Crossreactivities are found in cases of trichinellosis, hydatidosis, fasciolosis, ankylostomosis and strongyloidosis (40 to 83 %)

Contents

96 wells coated with antigens from adult females of Acanthocheilonema viteae.
TBS-Tween 50 ml
Washing solution 50 ml
Enzyme buffer 50 ml
Stop solution 25 ml
Negative control serum (rabbit) 200 µl
Cut-off serum (rabbit) 200 µl
Positive control serum (rabbit) 200 µl
Protein A - AP conjugate 300 µl
Alk. phosphatase substrate 20 tab.
Multipipette resevoir 25ml 1x
Frame for ELISA 8-wells holder 1x
Detailed protocol 

Ordering information

Acanthocheilonema viteae,
complete kit (96 assays) No. 9400

References

Gueglio, B. et al. (1995) Bulletin de la Société Française de Parasitologie, 13, 67-72.

Rahmah, N. et al (2001) Trans. R. Soc. Trop. Med. Hyg., 95, 280-284.

Houzé, S. et al. (2003) Société Française de Parasitologie.

European directive 98/79/EC registration: Click here.   Material Safety Data Sheet: Click here.
Technical sheet and instructions for article N° 9400, PDF format, 24 Ko: Click here.

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